DR: Hossam Farouk Moawad Ali
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Dr. Mohamed Sharaf EL-Din Zaky Badr
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- 2 biological safety cabints - 2 CO2 incubation. - Inverted microscope associated with camera - Every day every lab
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CO2- Incubator
https://www.nuaire.com/labgard-nu-540-class-ii-type-a2-biosafety-cabinet https://www.nuaire.com/products/co2-incubators
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Hettich - Universal 16
High-capacity, high-speed benchtop centrifuge, accommodates various tube systems from 0.4mL to 100mL, hematocrit capillaries, as well as microtiter plates, culture plates and cytology work, microprocessor controlled, Run up and run down times, input of acceleration and braking ramps ergonomically arranged, water protected foil pad for entering the parameters, speed RPM increments of 10, running time input in minutes and seconds, maximum of 99 minutes, impulse key, during centrifugation the actual values of the parameters are displayed, brushless frequency drive, 15V, 60Hz, 3.7A.
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Savant temperature cycler
gene amplification through temperature control and specific primer and enzyme
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7500 Fast Dx Real-Time PCR Instrument
The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performancerequired for high-quality results in a 96-well format.
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Eppendorf 5417R Refrigerated Microcentrifuge
• Refrigerated centrifuge • Spins up to 25000 x g for quick and efficient pelleting • Offers unsurpassed safety reliability and ease of use • Designed for use on the lab bench or in a cold room • Can accommodate a variety of microcentrifuge rotors
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32" Nucleic acid-pcr- workstation with filtration AirClean nucleic acid workstation with HEPA filter
ross-contamination during amplification of DNA and RNA can lead to results that are inaccurate. These results consume valuable time and expensive reagents. AirClean® PCR hoods protect sensitive reactions from airborne contaminants. Each system has a built-in 254 nm ultraviolet light for decontamination of consumables and equipment between amplifications. Combination workstations include vertical laminar flow action to prevent outside contaminants from entering the work area during manipulation.
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micro hematocrit centrifuge
The Haematospin 1400 is a highly dependable and easy to operate machine designed to give decades of solid service. Built from durable, high quality components it is the ideal tool in Haematology laboratories and clinics, producing reliable and accurate haematocrits year in year out. Its successful classic design is in widespread use world wide and we guarantee to give it total service and parts support into the future. A choice of rotors is available separately, to allow micro chemistry preparations and give the unit versatility in use.
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Cooling centrifuge
can handle a range of general-purpose processing including cell culture, microplate, bioproduction, blood separation, microbiology and many others. It features a convenient high 4L capacity and is available in both ventilated and refrigerated units. Refrigerated models are ideal for processing temperature-sensitive samples.
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ABX MICROS
https://www.horiba.com/us/en/medical/products/hematology/abx-micros/abx-micros-60-details/abx-micros-60-905/
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UPLC
UPLC is the result of holistic innovation that simultaneously re-engineered LC particle technology, column design, injectors, pumps, and detectors. The increased performance of sub-2-μm hybrid columns, used in conjunction with the ACQUITY UPLC® System's specialized ability to deliver mobile phase at high pressures with low dispersion, preserves the chromatographic benefits of such small-particle chemistries — producing sharper and more concentrated peaks. ACQUITY UPLC increases your throughput and reduces solvent use without compromising analytical results.
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Gel drier- d62
Mididry (D62) is the geldryer of choicefor drying multiple gels in parallel.The drying area is 30 cm x 40 cm.This standard size allows drying of upto 8 Minigels or 2 Multigel-Long or2 Maxigel resp. Model V15 •17 gels atthe same time.For drying large sequencing gels ormultiple large gels the Maxidry (D64),with a drying area of 40 cm x 60 cmis the best choice.Recommended vacuum pumps arelisted on the Membrane Pumps page.The use of special filter paper andcellophane results in perfect flat andtransparent gels for documentationand analysis (e. g. with the Biometra geldocumentation system).
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Hybridization oven- minioven
Hybridization incubators, also known as hybridization ovens, are used in life science and research laboratories for applications including nucleic acid hybridizations such as Northern (RNA) blots/hybridizations, Southern (DNA) blots/hybridizations, Western (protein) blots, and molecular biology assays. The blotting protocol is performed in glass hybridization tubes or hybridization bottles held in place by a tube rotator (rotisserie). We offer models with the additional functionality to operate as a shaker or rocker, and even as a UV crosslinker.
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Flourscent microscope-tri- with digital camera
https://www.leica-microsystems.com/science-lab/step-by-step-guide-to-fluorescence-microscopy/
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Flow cytometery
Navios EX Flow Cytometer The Navios EX flow cytometer offers a solution for advanced cytometry applications with workflows for high throughput laboratories. This is the next generation leveraging the laser and optics technology of our successful CytoFLEX research platform. Robust performance means peace of mind, because when analyzing important samples, every event matters. Up to 12 parameters for high complexity assays*; sensitivity and resolution; and quality, reproducible resultsWith 10 colors*, collect additional data points from each sample, Reduce the number of samples to prepare along with the possible pipetting errors
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Manual sequencer
“A DNA sequencing method is used to determine the nucleotides sequence- adenine, guanine, thymine and cytosine, on a DNA sequence orderly, using the fluorescent dye.”
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Gel documintionsystema
The unit automatically protects the user by switching off the UV lights when the door is opened A selection of filters (red, green and gold) are available to cover all major gel stains A UV protective viewing port allows the user to view their gel safely While imaging A rollout transilluminator tray for easy insertion and removal of gels A BioLite unit and filters can be added to any system for multiplex imaging capability Requires a computer and monitor (not included) For gel imaging and gel documentation.
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Gel documentation system
https://www.labbulletin.com/articles/New-Compact-Gel-Documentation-System-from-Syngene
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pulsed field electrophoresis
Rotating field electrophoresis (ROFE) Pulsed field electrophoresis with the Rotaphor system allows the separation of DNA beyond the 50 kb limit of conventional agarose electrophoresis.
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Stat Fax 2100 Microplate Reader
The Awareness Technology Stat Fax 2100 Microplate Reader is a compact, fully automatic reading unit. With its biochromatic optics, the reader is most ideal for in-vitro diagnostic use, computing the results of endpoint colorimetric assays.
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Ultrasonic homogenizer
https://www.coleparmer.com/tech-article/ultrasonic-homogenizer-selection-guide
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Hettich - Universal 16
High-capacity, high-speed benchtop centrifuge, accommodates various tube systems from 0.4mL to 100mL, hematocrit capillaries, as well as microtiter plates, culture plates and cytology work, microprocessor controlled, Run up and run down times, input of acceleration and braking ramps ergonomically arranged, water protected foil pad for entering the parameters, speed RPM increments of 10, running time input in minutes and seconds, maximum of 99 minutes, impulse key, during centrifugation the actual values of the parameters are displayed, brushless frequency drive, 15V, 60Hz, 3.7A.
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Biometra UNO II
The gold-plated sample block has been engineered for maximum performance and durability, utilizing a rapid heat transfer design of electroformed silver to maximize heating/cooling rates and goldplating for maximum durability. This allows the gold-plated silver 96-well 9700 system to be used in MAX Mode (5°C/sec average heating/cooling of block).
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Gene Amp PCR system 9700
The gold-plated silver sample block has been engineered for maximum performance and durability, utilizing a rapid heat transfer design of electroformed silver to maximize heating/cooling rates and goldplating for maximum durability. This allows the gold-plated silver 96-well 9700 system to be used in MAX Mode (5°C/sec average heating/cooling of block) and 9600 emulation mode. Please contact us for complete warranty, service contract and used/refurbished ABI 9700 system details.
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7500 FAST REAL TIME PCR
96-well format, high-speed thermal cycling, delivering high-quality results in less than 30 minutes • Offers robust high-resolution melting analysis • 5-color dye flexibility in a trusted 96-well format make amplification to 4 type of gene in one tube according to dye and probe dye • Many type of probe technique included as Taqman , Beacon , Scorpion- black hole and so on.
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ABI PRISM 310 Genetic Analyzer
The technologies used to map and sequence the human genome are also being used in leading research establishments to detect DNA mutations leading to genetic diseases such as muscular dystrophy, cystic fibrosis, HIV, and cancer susceptibility (1–3). The detection of mutations within many disease-related genes can be automated using fluorescent labeled fragments generated by PCR or cycle sequencing. One of Applied Biosystem’s main goals is to develop genetic analysis technologies to meet the needs of the clinical and research settings of the future
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Proflex PCR System
The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. And now you can access your ProFlex PCR System with Thermo Fisher Connect, giving you the freedom to design and securely upload your methods and monitor runs from any smartphone or desktop computer.
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Mini spin centrifuge
• Maximum speed/force with supplied rotor: 13,400rpm/12,100xG • 30-minute timer, settable in 15-sec. increments • Short-spin mode fixed at maximum speed
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PIPETTE- SET
The ultra light Eppendorf Research plus pipette meets the highest needs in precision and accuracy – combined with ultimate ergonomics and increased flexibility. The pipettes features a spring loaded tip cone, a secondary calibration option, an improved volume display and is fully autoclavable
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QUBIT 3.0 Fluorimeter
DNA & RNA Purification & Analysis,DNA Quantitation,Protein Assays and Analysis,Protein Biology,Protein Quantitation,RNA Quantitation.run on the Ion PGM™ Sequencer
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Ongoing Research & Projects
(MASRI-TISSUE CUlTURe Lab)
Read More(MASRI-Flowcytometry)
Read More(MASRI-ProteomiCS Unit)
Read More(MASRI-GenomicS Unit)
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Recent Publications
Effect of Conditioned and Co Culture Media from The Umbilical Cord Mesenchymal Stem Cells on Heptoma Cell Line (HuH7)
Author:3/8/2021
Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Ischaemic- Reperfused Hearts in Adult Rats with Established Chronic Kidney Disease.
Author:3/8/2021
In vitro comparative study between mechanically emulsified fat grafts and enzymatically emulsified fat grafts.
Author:3/8/2021
In vitro comparative study between mechanically emulsified fat grafts and enzymatically emulsified fat grafts
Author:3/8/2021
Assessment of healing in Calvarial bone defect by allogenic demineralized bone matrix and adipose derived stem cells (Experimental study).
Author:3/8/2021
Effect of bone marrow-derived mesenchymal stem cells transplantation on hypertension in experimental diabetic nephropathy
Author:3/8/2021
Ameliorative role of bone marrow mesenchymal stem cells and rutin against acetaminophen induced hepatotoxicity in adult male rats
Author:3/8/2021
The antiproliferative effect of Origanum majorana on human hepatocarcinoma cell line: suppression of NF-kB.
Author:3/8/2021
Histological study on the role of bone marrow derived mesenchymal stem cells on sciatic nerve and gastrocnemius muscle in a model of sciatic nerve crush injury in albino rat
Author:3/6/2021
Immunomodulatory capacity of the local mesenchymal stem cells transplantation after severe skeletal muscle injury in female rats
Author:3/6/2021
Autologous adipose-derived adult stem cell injection versus platelet-rich plasma injection in the treatment of rolling post acne scars
Author:3/6/2021
Therapeutic potential of mesenchymal stem cells on early and late experimental hepatic schistosomiasis model
Author:3/7/2021
Role of bone marrow mesenchaymal stem cells (MSCs) on restoration of fertility in male rats after exposure to endocrine disrupter
Author:3/2/2021
(Assessment of Interlukin-28B Polymorphism and Interferon Inducible Protein-10 level in Treatment Response Prediction of Hepatitis C Egyptian Patients (2020
Author:3/2/2021
(Thyroid Dysfunctions in Patients with Hepatocellular Carcinoma Treated by Transarterial Chemoembolization (2020
Author:3/3/2021
Genetic study on the effect of human mesenchymal stem cell on hepatoma cell line (HuH7) (2018
Author:3/5/2021
HLA-G expression in acute lymphoblastic leukemia: a significant prognostic tumor biomarker, Impact Factor 3.252
Author:2/26/2021
The antiproliferative effect of Origanum majorana on human hepatocarcinomatous cell line: suppression of NF-κ
Author:2/21/2021
Prof. Hesham Elghazaly
Head of MasriOn behalf of Ain Shams University and the MASRI team, I am honored to invite you to collaborate with this new hub for research and knowledge transfer...
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