FACULty of Medicine Ain ShamS ReSearch InSTITUTE - Flowcytometry Unit

FACULty of Medicine Ain ShamS ReSearch InSTITUTE - Flowcytometry Unit

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Unit Introduction:

Flow Cytometry: A technique for counting and examining microscopic particles, such as cells and chromosomes, by suspending those particles in a stream of fluid and passing them through an electronic detection device. This technology allows for a simultaneous multifactorial analysis of the physical and / or chemical properties of up to thousands of molecules per second. Cytometry is used routinely in many other applications in both scientific research and practice to diagnose diseases, especially leukemia, but it also has its own characteristics to separate and purify a specific clinical medical type.

 

The principle of flow cytometry: G that contains particles. A single (wavelength) laser beam is directed toward a flowing fluid, directing a number of sensors (detectors) to the point that the flowing liquid passes through the light beam: one sensor in the same direction as the FSC or Scatter Forward (and sensors), other perpendicular to the front light (SSC or Scatter Side), plus one or more fluorescent flash sensors

Activities:

    1. Post graduates (Master, PhD, Post Doc

Researchers) benefit from the highly equipped flow cytometry lab in getting consultation and laboratory assistance during completing their practical part of the thesis .

                 2. Post the 8th Gastrointestinal, Liver & UroOncology

Conference (IGILUC) hands on work shop (21-22) April 2019.

Achievements:

Contribution in a number of research papers in the following:

  • Immune-Modulatory Changes after Transplantation Therapy of Insulin Producing Cells Derived from Wharton’s Jelly Human Umbilical Cord- Mesenchymal Stem Cells in Diabetes Induced Rats.
  • Should human umbilical cord (wharton jelly) derived mesenchymal stem cells differentiate into insulin producing cells?
  • Immunomodulatory capacity of the local mesenchymal stem cells

        transplantation after severe skeletal muscle injury in female rats

  • Bone marrow derived-mesenchymal stem cells downregulate IL17A dependent IL6/ STAT3 signaling pathway in ccl 4-induced rat liver fibrosis
  • The Role of Stem Cells on the Ovarian Failure Induced by Busulfan in Female Albino Rat

 

 

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Prof. Hesham Elghazaly

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