“A DNA sequencing method is used to determine the nucleotides sequence- adenine, guanine, thymine and cytosine, on a DNA sequence orderly, using the fluorescent dye.”

DNA sequencing is required in every genetic lab because of its wide spectrum applications. Besides this, nowadays, it is most popular in prenatal genetic screening and preimplantation genetic studies.

However, the entire sequencing assay is dependable on the manufacturer, all utilities, kits, reagents and instrument are provided by manufacturer itself thus additional optimization and experimentation are restricted.

We have to depend on the SOPs and protocol provided by the supplier only.